Composite

Part:BBa_K5206004:Design

Designed by: Yaoyao Liang   Group: iGEM24_Nanjing-BioX   (2024-09-30)


alsR-Palsl-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2355
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1640


Design Notes

In order to reduce the metabolic burden of two separate plasmids on the host cell, this study adopted an integrative strategy to integrate all the components critical for biosensor function into a single plasmid vector to optimise the compactness of the genetic circuit and the efficiency of expression. This was done to test the performance of the integrated biosensor in response to specific metabolites and to assess whether its potential for synthetic biology applications was optimised.


Source

None

References